FOXO4-DRI 5mg

FOXO4-DRI is a synthetic D-retro-inverso peptide: the chain is assembled entirely from D-amino acids in reversed sequence order relative to the parent L-peptide, so that side-chain topology approximates the parent while the backbone direction is inverted. The construct joins a segment derived from the FOXO4 forkhead domain to a polybasic cell-penetrating sequence. It is supplied as a lyophilized solid.

The designed target is the protein–protein interface between FOXO4 and the transactivation domain of p53. Interface occupancy is quantified in vitro by fluorescence-polarisation and competition binding assays using recombinant FOXO4 forkhead domain and labelled p53-derived probe peptides. Co- immunoprecipitation and AlphaScreen formats in cell lysates give an orthogonal readout of complex formation. Characterisation is reported at the level of the measured binding constant and stops there.

Primary literature addresses the chemistry of D-retro-inverso peptide synthesis and the structural basis of the FOXO4–p53 interface, including fluorescence-polarisation and co-immunoprecipitation methodology. Search results and cited literature do not establish the performance of a commercial lot or a use recommendation.