MOTS-c 40mg

MOTS-c is a synthetic 16-residue peptide whose sequence is encoded by a short open reading frame within the mitochondrial 12S ribosomal RNA gene. The chain is linear and unmodified at both termini, contains two methionine residues, one tryptophan and two tyrosines, and carries three arginines and one lysine that make it strongly cationic at neutral pH. It contains no cysteine and forms no disulfide bond.

MOTS-c is examined in cell-free and cell-culture systems against AMP-activated protein kinase (AMPK) and the folate-cycle enzyme MTHFD1. AMPK phosphorylation at Thr172 is quantified by immunoblot in cultured cell lysates, expressed as phospho-to-total signal ratio. Direct binding to MTHFD1 is measured by pull-down from cell lysate and by surface plasmon resonance against immobilised enzyme, with association and dissociation constants derived from the sensorgram. Readouts terminate at phosphorylation-site signal and binding-constant values.

Primary literature on this molecule addresses the identification of its open reading frame, its solid-phase synthesis and analytical characterisation, and cell-free and cell-culture enzyme-binding chemistry. Search results and cited literature do not establish the performance of a commercial lot or a use recommendation.