Vitamin B-12 10mg/10mL

Cyanocobalamin is a cobalt-centred corrinoid and one of the vitamin B-12 vitamers, in which a cyano group occupies the upper axial coordination site of the central cobalt ion. The corrin macrocycle is linked through an aminopropanol arm to a 5,6-dimethylbenzimidazole nucleotide that furnishes the lower axial ligand. It is a small-molecule coordination compound, not a peptide, and is supplied here in aqueous solution.

Cyanocobalamin is the precursor of the two cobalamin cofactor forms assayed in vitro: methylcobalamin at methionine synthase (MTR) and 5′-deoxyadenosylcobalamin at methylmalonyl-CoA mutase (MMUT). Cofactor occupancy and enzyme kinetics are measured in recombinant-enzyme preparations by coupled spectrophotometric and radiometric assays. Association with the cobalamin transport proteins transcobalamin and intrinsic factor is quantified in solution-phase binding assays. Reported endpoints are association constants and kinetic parameters in these defined systems.

Primary literature covers the coordination chemistry and crystallography of the corrin macrocycle, the spectrophotometric and chromatographic determination of cobalamin vitamers, and the enzymology of the cobalamin-dependent enzymes named above. Search results and cited literature do not establish the performance of a commercial lot or a use recommendation.